Sistema de Eventos Acadêmicos da UFMT, XI Mostra da Pós-Graduação

Tamanho da fonte: 
Early malprogramming of high-caloric diet reference and hyperphagia in later life due to lactating undernutrition
Ginislene Dias Souza Miranda, Thalyne Aparecida Leite de Lima, Bárbara Letícia Antonio Membrive, Hercules de Oliveira Costermani, Camila Luiza dos Santos Rodrigues Ricken, Sara Santos dos Reis, Raul Evangelista de Almeida, Jocemara Patrícia Silva de Souza Parrela, Júlio Cezar De Oliveira

Última alteração: 09-10-19

Resumo


Background: Energy metabolism can be impaired earlier in pup’s life due to undernutrition in maternal environment. It may be associated with the risk of developing metabolic syndrome in adulthood. Herein we evaluate the effect of maternal feeding restriction during the first 2/3 of lactation on feeding behavior and biometric profile of male rat offspring. Method: Restricted mothers were fed a half of the diet amount offered to control mothers during the first two weeks of lactation (FR50 group).While control rats (CONT group) were fed ad libitum. Rat offspring’s body weight gain and food intake were quantified every 2 days. Food preference (rodent chow versus high-caloric diet, HCD) was evaluated over a 10-day period. At 90 days of age, food intake was evaluated during the dark cycle (from 18h to 23h and 6h). At 100 days of age, rats were euthanized to remove adipose and skeletal muscle tissues for quantification of biometric parameters. The Ethics Committee approved the experimental procedures (protocol #:23108.709618-2015-21). Results: Compared to CONT rats, the body weight of FR50 rats was reduced by 14.3% (n=17 rats, P<0.001), while food intake during the experimental period was increased by 8.2% (n=3 litter, P<0.05). Regarding food preference, even the CONT rats consumed 16.3% more HCD, it was not statistically different, on the other hand the FR50 rats ingested 96.8% more HCD (P<0.001). In the dark cycle, the food intake of FR50 rats for the first 5 hours was similar to control rats; however, overnight it was increased by 13.30% (n=5, P<0.01). In relation to the CONT rats, the main fat pad stores from FR50 rats were reduced (retroperitoneal: –22.1% and mesenteric: –20.3%; n=16, P<0.001). The extensor digitorum longus muscle was not different between groups. Conclusion: Maternal dietary restriction during the first phase of lactation imprints a thin phenotype, even though associated with hyperphagia and high preference for a HCD. It implies FR50 rats as a vulnerable rat model to develop metabolic diseases.

 


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Keywords: Metabolic programming, perinatal undernutrition, high-caloric diet, hyperphagia, metabolic syndrome, suckling phase.