Sistema de Eventos Acadêmicos da UFMT, XI Mostra da Pós-Graduação

Tamanho da fonte: 
Synthesis and larvicidal activity of indole derivatives against Aedes aegypti (Diptera: Culicidae)
JANAINA ROSA DE SOUSA

Última alteração: 08-10-19

Resumo


In light of the challenges to control  Aedes  aegypti  and the critical role that it plays as arbovirus vector, it  is imperative to adopt  strategies that  provide fast, efficient and environmentally  safe control of  the insect  population. In the present study, we synthesized  six  indole  derivatives  (C1‐C6)  and  examined  their  larvicidal  activity  and persistence  against  Ae.  aegypti  larvae, as well as their toxicity towards Raw 264.7 macrophages, Vero cells,  Chlorella  vulgaris  BR017,  Scenedesmus  obliquus  BR003, Caenorhabditis  elegans  N2 and  Galleria  mellonella. Among the bioactive compounds (C1, C2, C4 and C5), C2 exerted the strongest larvicidal activity against  Ae.  aegypti, with LC50  =  1.5  μg/ml (5.88  µM) and LC90  =  2.4  μg/ml (9.50  µM), indicating that the presence of  chlorine or bromine groups in the aromatic  ring improved the larvicidal activity of the indole derivatives. C1, C2, C4 and C5 did not reduce viability of RAW 264.7  macrophages,  Vero  cells,  C.  elegans  N2  and  G.  mellonella.  Compounds  C1,  C2 and C5 did not affect the growth of  C.  vulgaris  BR017 and  S.  obliquus  BR003. Analysis of larvicidal persistence under laboratory conditions revealed that the effect of compounds C1, C2, C4 and C5 lasted for 30  days and caused 100% of larvae mortality within few  hours. Altogether, our findings demonstrate that  the indole derivatives C1, C2, C4 and C5 effectively  control  Ae.  aegypti  larvae  population,  without  clear signs of toxicity to mammalian cells, algae,  C.  elegans  and  G.  mellonella.

Palavras-chave


Caenorhabditis elegans, environmental safety, Galleria mellonella, synthesis, toxicity