Sistema de Eventos Acadêmicos da UFMT, X Mostra da Pós-Graduação: Direitos Humanos, trabalho coletivo e redes de pesquisa na Pós Graduação

Tamanho da fonte: 
PERINATAL FOOD-RESTRICTION INCREASES BROWN ADIPOSE TISSUE PREVENTING OBESITY IN MALE RAT-OFFSPRING
Ginislene Dias Souza Miranda, Thalyne Aparecida Leite Lima, Júlia Cristina Facchi, Lucas Ryba Oliveira, Sara Santos Reis, Hercules de Oliveira Costermani, Júlio Cezar de Oliveira

Última alteração: 05-10-18

Resumo


Nutritional insults during perinatal life impair energy metabolism in later life. In this regards, unbalanced autonomous nervous system, especially high parasympathetic tonus can contribute to obesity onset. We hypothesized that sympathetic hyperactivity may be preventing obesity in rat-offspring whose dams underwent food restriction in lactation. Thus, we aimed to assess brown adipose tissue (BAT) as hallmark for the sympathetic function in this rat-model. At birth, pups were weighted and litter size adjusted to eight pups per dam. The control dams were fed ad libitum throughout lactation, whereas food restricted group received just 50% of diet offered to control dams (FR50 group) until the day 14th of lactation. At 21-days-old, rat-offspring was weaned, and then fed ad libitum until 100-days-old, where both body weight and food intake were assessed each two days. At 100-days-old, the food intake during dark-cycle (from 6 PM to 11 PM and 6 PM to 6 AM) was evaluated. Body weight and mesenteric fat pad, as well as lean mass (extensor digitorum longus, EDL, skeletal muscle) were used to assess the body composition. The weight of BAT was quantified as a marker for sympathetic nervous activity. When compared to control rats, the assessment of area under the curve (AUC) of body weight gain from FR50 rat-offspring displayed a reduction of 9.04%, while the AUC of food intake was increased by 10.39% (n=20, P<0.01). At dark-cycle, the food intake of FR50 rats in the first 4h was similar to control rats; however, at overnight, it was increased by 13.30% (n=5, P<0.01). In relation to control group, the body composition markers in FR50 rats were reduced (mesenteric fat pad, –36.11%, n=14, P<0.05; and EDL, –52.37%, n=20, P<0.01). By other hand, BAT was 38.13% higher in FR50 rats (n=20, P<0.001) than control. Calorie-restriction, just at the first phase of lactation, implies a lean phenotype, even associated with a paradoxical hyperphagia, or in other words “obesity resistance”. It may be due to high BAT that implicates hyperactivity of the sympathetic nervous system blocking body weight gain.

Palavras-chave


Food-restriction; hyperphagia; lactation; malprogramming; thermogenesis