Sistema de Eventos Acadêmicos da UFMT, XI Mostra da Pós-Graduação

Tamanho da fonte: 
Intrauterine food-restriction malprograms obesity and associated metabolic dysfunction in young rats
Jocemara Souza Parrela

Última alteração: 09-10-19


Background: Intrauterine malnutrition has been put out into the field of metabolic diseases as a strong programming factor to high vulnerability to metabolic diseases throughout life. In the current study, we aimed to evaluate the effect of intrauterine malnutrition on body composition and the biochemical and hormonal parameters of the weaned rat offspring. Methods: Wistar female rats were mated and the pregnancy assessed by the vaginal smear observation, where sperm were used to mark pregnancy start. On the 14th day of pregnancy, amount of food was reduced by 50% (FR50 group) until delivery, while the control group (CONT group) was fed ad libitum. At birth, the pups were weighed and litter size adjusted to 8 pups per mother. Body weight gain was quantified every 2 days throughout suckling period. At weaning, overnight fasting rat offspring were euthanized to remove fat stores (mesenteric, retroperitoneal and periepididymal) to quantify body composition and blood removal for biochemical and hormonal quantification. Peripheral insulin sensitivity was assessed by the TyG index calculation, where fasting glycemia and triglyceridemia are used to do that. The Ethics Committee approved the experimental procedures (protocol number: 23.108724433/2017-16). Results: At birth, FR50 rats were smaller (–19.43%, P<0.001) than CONT rats. By other hand, the FR50 rats’ body weight gain, over the suckling period, was increased around of 62% (P<0.001), as well as higher fat pad stores (mesenteric, 83.33%; retroperitoneal, 180% and periepididymal, 63.27%; P<0.001). At weaning, compared to CONT rats, the FR50 rats presented hyperglycemia (+20.78%, P<0.05), hypertriglyceridemia (+75.41%, P<0.01), hypergrelinemia (+81.40%, P<0.05) and hyperleptinemia (+74.55%, P<0.001). Indeed, in relation to the CONT group, the TyG values was increased in FR50 rats (+7.01%, P<0.001), characterizing insulin resistance. Conclusion: Intrauterine malnutrition programs early obesity and insulin resistance development in rats, which is associated to changes in energy-balance controlling hormones.



Metabolic programming; energy homeostasis; insulin resistance.